Establishment of an estrogen responsive rat pituitary cell sub-line MtT/E-2

被引：15

作者：

Fujimoto, N ^{[1
]}

Maruyama, S ^{[1
]}

Ito, A ^{[1
]}

机构：

[1] Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Canc Res, Minami Ku, Hiroshima 7348553, Japan

来源：

ENDOCRINE JOURNAL | 1999年 / 46卷 / 03期

关键词：

rat pituitary cell line; estrogen; PTTG; c-myc; cyclin D1;

D O I：

10.1507/endocrj.46.389

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A rat pituitary tumor sub-line MtT/E-2 was established from a rat pituitary tumor cell line MtT/E. Its growth was found to depend on the presence of estradiol (E-2) in culture media at 10(-13)-10(-9) M, whereas the original cell line MtT/E proliferated autonomously. The recently discovered PTTG (pituitary tumor transforming gene) is highly expressed in this cell line, although not regulated by E-2. On the other hand, E-2 induced c-myc and cyclin D1 proteins in MtT/E-2, which contains a lot (220 +/- 18 fmol/mg protein) of estrogen receptor demonstrated by RT-PCR analysis to be predominantly alpha type. MtT/E-2 secretes growth hormone which is, interestingly, regulated by retinoic acid and dexamethasone rather than thyroid hormones.

引用

页码：389 / 396

页数：8

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