Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and β-galactosidases

被引:147
作者
Gee, KR [1 ]
Sun, WC [1 ]
Bhalgat, MK [1 ]
Upson, RH [1 ]
Klaubert, DH [1 ]
Latham, KA [1 ]
Haugland, RP [1 ]
机构
[1] Mol Probes Inc, Eugene, OR 97402 USA
关键词
phosphatase; beta-galactosidase; 4-methylumbelliferone; enzyme assays; high-thoughput screening;
D O I
10.1006/abio.1999.4202
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fluorogenic substrates based on 4-methylumbelliferone (4-MU) have been widely used for the detection of phosphatase and glycosidase activities. One disadvantage of these substrates, however, is that maximum fluorescence of the reaction product requires an alkaline pH, since 4-MU has a pK(a) approximate to 8. In an initial screening of five phosphatase substrates based on fluorinated derivatives of 4-MU, all with pK(a) values lower than that of 4-MU, we found that one substrate, 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP), was much improved for the detection of acid phosphatase activity, When measured at the preferred acid phosphatase reaction pH (5.0), DiFMUP yielded fluorescence signals that were more than 16-fold higher than those of 4-methylumbelliferyl phosphate (MUP). DiFMUP was also superior to MUP for the detection of protein phosphatase 1 activity at pH 7 and was just as sensitive as MUP for the detection of alkaline phosphatase activity at pH 10. A beta-galactosidase substrate was also prepared based on 6,8-difluoro 4-methylumbelliferone. This substrate, 6,8-difluoro-4-methylumbelliferyl beta-D-galactopyranoside (DiFMUG), was found to be considerably more sensitive than the commonly used substrate 4-methylurnbelliferyl beta-D-galactopyranoside (MUG), for the detection of beta-galactosidase activity at pH 7. DiFMUP and DiFMUG should have great utility for the continuous assay of phosphatase and beta-galactosidase activity, respectively, at neutral and acid pH. (C) 1999 Academic Press.
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页码:41 / 48
页数:8
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