Purification and characterization of two extracellular alkaline phosphatases from a psychrophilic Arthrobacter isolate

被引:9
作者
dePrada, P [1 ]
Brenchley, JE [1 ]
机构
[1] PENN STATE UNIV,DEPT BIOCHEM & MOL BIOL,UNIVERSITY PK,PA 16802
关键词
D O I
10.1128/AEM.63.7.2928-2931.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two extracellular, heat-labile alkaline phosphatases were purified from a psychrophilic Arthrobacter isolate, D10. The enzymes were active over different pH ranges, used distinct substrates, and had different kinetic properties. Each enzyme reacted specifically to its own antibody during immunoblot analysis. One had both monophosphatase and diesterase activities.
引用
收藏
页码:2928 / 2931
页数:4
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