Identification of bluetongue virus serotype 2 (Corsican strain) by reverse-transcriptase PCR reaction analysis of segment 2 of the genome

被引:45
作者
Zientara, S [1 ]
Sailleau, C [1 ]
Dauphin, G [1 ]
Roquier, C [1 ]
Rémond, EM [1 ]
Lebreton, F [1 ]
Hammoumi, S [1 ]
Dubois, E [1 ]
Agier, C [1 ]
Merle, G [1 ]
Bréard, E [1 ]
机构
[1] Agence Francaise Secur Sanitaire Aliments Alfort, F-94703 Maisons Alfort, France
关键词
D O I
10.1136/vr.150.19.598
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In October 2000, bluetongue virus was detected on the French island of Corsica. The disease was also reported in Sardinia, Calabria, Sicily and on the Spanish islands of Majorca and Minorca. This paper describes the use of molecular techniques for a rapid identification and serotype determination of serotype 2 of the virus. The nucleotide sequences of segments 2 and 7 of the genome of the Corsican strain were determined and its phylogenetic relationships are described.
引用
收藏
页码:598 / 601
页数:4
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