Changes in gene expression during male meiosis in Petunia hybrida

被引:27
作者
Cnudde, Filip
Hedatale, Veena
de Jong, Hans
Pierson, Elisabeth S.
Rainey, Daphne Y.
Zabeau, Marc
Weterings, Koen
Gerats, Tom
Peters, Janny L.
机构
[1] Radboud Univ Nijmegen, Inst Wetland & Water Res, Dept Expt Bot, Sect Plant Genet, NL-6525 ED Nijmegen, Netherlands
[2] Univ Wageningen & Res Ctr, Genet Lab, NL-6703 HA Wageningen, Netherlands
[3] Radboud Univ Nijmegen, Dept Gen Instrumentat, NL-6525 ED Nijmegen, Netherlands
[4] Keygene NV, NL-6700 AE Wageningen, Netherlands
[5] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium
关键词
anther; cDNA-AFLP; expression analysis; meiosis; Petunia hybrida; pollen mother cell; recombination; transcript profiling;
D O I
10.1007/s10577-006-1099-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We analyzed changes in gene expression during male meiosis in Petunia by combining the meiotic staging of pollen mother cells from a single anther with cDNA-AFLP transcript profiling of mRNA from the synchronously developing sister anthers. The transcript profiling experiments focused on the identification of genes with a modulated expression profile during meiosis, while premeiotic archesporial cells and postmeiotic microspores served as a reference. About 8000 transcript tags, estimated at 30% of the total transcriptome, were generated, of which around 6% exhibited a modulated gene expression pattern at meiosis. Cluster analysis revealed a transcriptional cascade that coincides with the initiation and progression through all stages of the two meiotic divisions. Fragments that exhibited high expression specifically during meiosis I were characterized further by sequencing; 90 out of the 293 sequenced fragments showed homology with known genes, belonging to a wide range of gene classes, including previously characterized meiotic genes. In-situ hybridization experiments were performed to determine the spatial expression pattern for five selected transcript tags. Its concurrence with cDNA-AFLP transcript profiles indicates that this is an excellent approach to study genes involved in specialized processes such as meiosis. Our data set provides the potential to unravel unique meiotic genes that are as yet elusive to reverse genetics approaches.
引用
收藏
页码:919 / 932
页数:14
相关论文
共 55 条
[1]   THE EFFECT ON MEIOTIC SYNAPSIS OF A RECOMBINATION MODULATOR IN PETUNIA-HYBRIDA [J].
ABIRACHEDDARMENCY, M ;
TARENGHI, E ;
DEJONG, JH .
GENOME, 1992, 35 (03) :443-453
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   The spindle checkpoint [J].
Amon, A .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1999, 9 (01) :69-75
[4]   Meiotic cytology and chromosome behaviour in wild-type Arabidopsis thaliana [J].
Armstrong, SJ ;
Jones, GH .
JOURNAL OF EXPERIMENTAL BOTANY, 2003, 54 (380) :1-10
[5]   Asy1, a protein required for melotic chromosome synapsis, localizes to axis-associated chromatin in Arabidopsis and Brassica [J].
Armstrong, SJ ;
Caryl, AP ;
Jones, GH ;
Franklin, FCH .
JOURNAL OF CELL SCIENCE, 2002, 115 (18) :3645-3655
[6]  
Armstrong SJ, 2001, J CELL SCI, V114, P4207
[7]   Homolog interaction during meiotic prophase I in Arabidopsis requires the SOLO DANCERS gene encoding a novel cyclin-like protein [J].
Azumi, Y ;
Liu, DH ;
Zhao, DZ ;
Li, WX ;
Wang, GF ;
Hu, Y ;
Ma, H .
EMBO JOURNAL, 2002, 21 (12) :3081-3095
[8]   Quantitative cDNA-AFLP analysis for genome-wide expression studies [J].
Breyne, P ;
Dreesen, R ;
Cannoot, B ;
Rombaut, D ;
Vandepoele, K ;
Rombauts, S ;
Vanderhaeghen, R ;
Inzé, D ;
Zabeau, M .
MOLECULAR GENETICS AND GENOMICS, 2003, 269 (02) :173-179
[9]   Transcriptome analysis during cell division in plants [J].
Breyne, P ;
Dreesen, R ;
Vandepoele, K ;
De Veylder, L ;
Van Breusegem, F ;
Callewaert, L ;
Rombauts, S ;
Raes, J ;
Cannoot, B ;
Engler, G ;
Inzé, D ;
Zabeau, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14825-14830
[10]   Severe developmental defects, hypersensitivity to DNA-damaging agents, and lengthened telomeres in Arabidopsis MRE11 mutants [J].
Bundock, P ;
Hooykaas, P .
PLANT CELL, 2002, 14 (10) :2451-2462