共 94 条
Embryonic stem cell-derived neurons form functional networks in vitro
被引:46
作者:

Ban, Jelena
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h-index: 0
机构: Sch Adv Int Studies, I-34014 Trieste, Italy

Bonifazi, Paolo
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机构: Sch Adv Int Studies, I-34014 Trieste, Italy

Pinato, Giulietta
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h-index: 0
机构: Sch Adv Int Studies, I-34014 Trieste, Italy

Broccard, Frederic D.
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机构: Sch Adv Int Studies, I-34014 Trieste, Italy

Studer, Lorenz
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机构: Sch Adv Int Studies, I-34014 Trieste, Italy

Torre, Vincent
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h-index: 0
机构: Sch Adv Int Studies, I-34014 Trieste, Italy

Ruaro, Maria Elisabetta
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h-index: 0
机构: Sch Adv Int Studies, I-34014 Trieste, Italy
机构:
[1] Sch Adv Int Studies, I-34014 Trieste, Italy
[2] Mem Sloan Kettering Canc Ctr, Lab Stem Cell & Tumor Biol, Div Neurosurg, New York, NY 10021 USA
[3] Mem Sloan Kettering Canc Ctr, Dev Biol Program, New York, NY 10021 USA
来源:
关键词:
ES cells;
in vitro differentiation;
neuronal network;
hippocampus;
D O I:
10.1634/stemcells.2006-0246
中图分类号:
Q813 [细胞工程];
学科分类号:
摘要:
Embryonic stem (ES) cells provide a flexible and unlimited source for a variety of neuronal types. Because mature neurons establish neuronal networks very easily, we tested whether ES-derived neurons are capable of generating functional networks and whether these networks, generated in vitro, are capable of processing information. Single-cell electrophysiology with pharmacological antagonists demonstrated the presence of both excitatory and inhibitory synaptic connections. Extracellular recording with planar multielectrode arrays showed that spontaneous bursts of electrical activity are present in ES-derived networks with properties remarkably similar to those of hippocampal neurons. When stimulated with extracellular electrodes, ES-derived neurons fired action potentials, and the evoked electrical activity spread throughout the culture. A statistical analysis indicated that ES-derived networks discriminated between stimuli of different intensity at a single trial level, a key feature for an efficient information processing. Thus, ES-derived neurons provide a novel in vitro strategy to create functional networks with defined computational properties.
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页码:738 / 749
页数:12
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