Enhancement of sensitivity using gold nanorods-Antibody conjugator for detection of E. coli O157:H7

被引：52

作者：

Eum, Nyeon-Sik ^{[2
,4
]}

Yeom, Se-Hyuk ^{[1
]}

Kwon, Dae-Hyuk ^{[3
]}

Kim, Hak-Rin ^{[1
]}

Kang, Shin-Won ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Sch Elect Engn & Comp Sci, Taegu 702701, South Korea

[2] Keimyung Univ, Ctr IT Med Device Convergence, Taegu 704701, South Korea

[3] Kyungil Univ, Sch Elect Informat & Commun Engn, Gyeongbuk 712701, South Korea

[4] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2010年 / 143卷 / 02期

关键词：

Nano-SPR; Surface plasmon resonance; Gold nanorod; E. coli O157:H7; Spreeta; SURFACE-PLASMON RESONANCE; CELLS;

D O I：

10.1016/j.snb.2009.09.054

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, GNRs (gold nanorods) were used to enhance the sensitivity of the biosensor for Escherichia coli O157:H7 in Surface plasmon resonance (SPR) system. GNRs were designed and fabricated through seed-medicated growth and Surface activation by alkanethiols for the attaching antibody. Antibodies for E coli O157:H7 were immobilized on GNRs and measured by using UV-visible spectroscopy and sandwich assays were carried out to detect E. coli O157:H7 using Spreeta. This experiment is expected to play a key role in increasing sensitivity in other biosensor experiments and photothermal therapy. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：784 / 788

页数：5

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