Quantitative DNA slot blot analysis: inhibition of DNA binding to membranes by magnesium ions

被引：45

作者：

Kube, DM

Srivastava, A

机构：

[1] INDIANA UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,INDIANAPOLIS,IN 46202

[2] INDIANA UNIV,SCH MED,DEPT MED,DIV HEMATOL ONCOL,INDIANAPOLIS,IN 46202

[3] WALTHER CANC INST,WALTHER ONCOL CTR,INDIANAPOLIS,IN 46202

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 16期

关键词：

D O I：

10.1093/nar/25.16.3375

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Titers of wild-type and recombinant adeno-associated viruses are routinely determined by DNA slot blot analysis. The binding of viral DNA to nylon membranes was found to be inhibited by magnesium ions, which are critical components of the DNase I digestion carried out prior to slot blot analysis. Mg2+ ions also interfered with the adsorption of plasmid DNA to nylon and nitrocellulose membranes. These observations yield practical insights into the poorly understood mechanisms by which DNA molecules are retained on solid supports.

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页码：3375 / 3376

页数：2

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