A new approach for the study of cationic lipid-DNA complexes by energy dispersive X-ray diffraction

被引：18

作者：

Caracciolo, G

Caminiti, R ^{[1
]}

Natali, F

Castellano, AC

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, IT-00185 Rome, Italy

[2] Univ Roma La Sapienza, Ist Nazl Fis Nucl, IT-00185 Rome, Italy

[3] Univ Roma La Sapienza, Dipartimento Fis, IT-00185 Rome, Italy

[4] Inst Max Von Laue Paul Langevin, OGG, INFM, F-38042 Grenoble 9, France

来源：

CHEMICAL PHYSICS LETTERS | 2002年 / 366卷 / 3-4期

关键词：

D O I：

10.1016/S0009-2614(02)01454-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

X-ray diffraction (XRD) studies on the cationic liposomes (CL)-DNA complexes currently used in gene delivery have provided detailed structural informations on these compact, ordered self-assemblies sheding light on the poorly understood structure-activity relationship. Among these, the experiments carried out by using a synchrotron radiation source have showed an experimental resolution remarkably better than that achieved one by traditional in house apparatuses. Here we show a new experimental approach for the study of CL-DNA complexes, based on the employment of silicon wafers as substrates, which allows to obtain high-resolution structural informations by energy dispersive X-ray diffraction (EDXD). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：200 / 204

页数：5

共 19 条

[1] A new technique for the study of phase transitions by means of energy dispersive X-ray diffraction. Application to polymeric samples [J].