A restriction fragment length polymorphism assay that differentiates human N-acetyltransferase-1 (NAT1) alleles

被引：48

作者：

Deitz, AC ^{[1
]}

Doll, MA ^{[1
]}

Hein, DW ^{[1
]}

机构：

[1] UNIV N DAKOTA,SCH MED & HLTH SCI,DEPT PHARMACOL & TOXICOL,GRAND FORKS,ND 58202

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 253卷 / 02期

关键词：

D O I：

10.1006/abio.1997.2379

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Currently there is much interest in the N-acetyltransferase-1 (NAT1) genetic polymorphism and its relationship to cancer. Previous studies have described methods to distinguish NAT1 alleles through polymer ase chain reaction (PCR) restriction fragment length polymorphism (RFLP) and/or allele-specific amplification. However, these methods detect at most only four of the NAT1 alleles identified in human populations. In this paper we describe a PCR-RFLP-based assay that differentiates among eight human NAT1 alleles (NAT1*3, *4, *5, *10, *11, *14, *15, *16). This method should prove useful in molecular epidemiological studies investigating associations between NAT1 genotype and cancer. (C) 1997 Academic Press.

引用

页码：219 / 224

页数：6

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