DNA methylation immediately adjacent to active histone marking does not silence transcription

被引：17

作者：

Brinkman, Arie B. ^{[1
]}

Pennings, Sebastiaan W. C. ^{[1
]}

Braliou, Georgia G. ^{[1
]}

Rietveld, Luc E. G. ^{[1
]}

Stunnenberg, Hendrik G. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Nijmegen Ctr Mol Life Sci 191, Dept Biol Mol, NL-6500 HB Nijmegen, Netherlands

来源：

NUCLEIC ACIDS RESEARCH | 2007年 / 35卷 / 03期

关键词：

D O I：

10.1093/nar/gkl1014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Active promoters generally contain histone H3/H4 hyperacetylation and tri-methylation at H3 lysine 4, whereas repressed promoters are associated with DNA methylation. Here we show that the repressed erythroid-specific carbonic anhydrase II (CAII) promoter has active histone modifications localized around the transcription start, while high levels of CpG methylation are present directly upstream from these active marks. Despite the presence of active histone modifications, the repressed promoter requires hormone-induced activation for efficient preinitiation complex assembly. Transient and positional changes in histone H3/H4 acetylation and local changes in nucleosome density are evident during activation, but the bipartite epigenetic code is stably maintained. Our results suggest that active histone modifications may prevent spreading of CpG methylation towards the promoter and show that repressive DNA methylation immediately adjacent to a promoter does not necessarily repress transcription.

引用

页码：801 / 811

页数：11

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