The expression of CD40-CD40L and activities of matrix metalloproteinases in atherosclerotic rats

被引：16

作者：

Wu, M ^{[1
]}

Li, YG ^{[1
]}

机构：

[1] Shantou Univ, Coll Med, Affiliated Hosp 1, Dept Cardiol, Shantou 515041, Guangdong, Peoples R China

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2006年 / 282卷 / 1-2期

关键词：

adhesion factor; atherosclerosis; CD40; CD40L; matrix metalloproteinase;

D O I：

10.1007/s11010-006-1741-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study investigated the expression of CD40, CD40 ligand (CD40L) and matrix metalloproteinases (MMPs) in dietary-induced atherosclerosis in rats. Wister rats were fed with high cholesterol diet (As group, n = 6) or with normal diet (N group, n = 6). Blood cells that express CD40 and CD40L were sorted by flow cytometry, the MMP-2 and MMP-9 were measured by zymography method. The morphological locations of MMP-2 and MMP-9 in the aorta were studied with immunohistochemistry and by microscopy. The results showed that the expression of CD40, CD40L and matrix metalloproteinase were higher in As group than those in control group. The MMP-2 and MMP-9 were positive in As group but negative in control group by immunohistochemistry study. Our results suggest that the expression of CD40 and CD40L in the blood cells and the activities of MMP-2 and MMP-9 in plasma were higher in As group than those in Normal group, indicating that they may contribute to the formation of atherosclerosis.

引用

页码：141 / 146

页数：6

共 23 条

[1] Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α [J].

Dardik, A ;

Yamashita, A ;

Aziz, F ;

Asada, H ;

Sumpio, BE .

JOURNAL OF VASCULAR SURGERY, 2005, 41 (02) :321-331

[2] Reactive oxygen species, metalloproteinases, and plaque stability [J].

Davies, MJ .

CIRCULATION, 1998, 97 (24) :2382-2383

[3]

Eckart Robert E, 2004, J Interv Cardiol, V17, P27, DOI 10.1111/j.1540-8183.2004.00289.x

[4]

Ferroni P, 2003, J INVEST MED, V51, P295, DOI 10.1136/jim-51-05-17

[5] Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas [J].

Forsyth, PA ;

Wong, H ;

Laing, TD ;

Rewcastle, NB ;

Morris, DG ;

Muzik, H ;

Leco, KJ ;

Johnston, RN ;

Brasher, PMA ;

Sutherland, G ;

Edwards, DR .

BRITISH JOURNAL OF CANCER, 1999, 79 (11-12) :1828-1835

[6] Impaired CD40L signaling is a cause of defective IL-12 and TNF-α production in Sezary syndrome:: circumvention by hexameric soluble CD40L [J].

French, LE ;

Huard, B ;

Wysocka, M ;

Shane, R ;

Contassot, E ;

Arrighi, JF ;

Piguet, V ;

Calderara, S ;

Rook, AH .

BLOOD, 2005, 105 (01) :219-225

[7] Apoptotic cell death during ischemia/reperfusion and its attenuation by antioxidant therapy [J].

Galang, N ;

Sasaki, H ;

Maulik, N .

TOXICOLOGY, 2000, 148 (2-3) :111-118

[8] Requirement for CD40 ligand in costimulation induction, T cell activation, and experimental allergic encephalomyelitis [J].

Grewal, IS ;

Foellmer, HG ;

Grewal, KD ;

Xu, JC ;

Hardardottir, F ;

Baron, JL ;

Janeway, CA ;

Flavell, RA .

SCIENCE, 1996, 273 (5283) :1864-1867

[9]

Jiang X, 2004, CHINESE MED J-PEKING, V117, P1825

[10] LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells [J].

Li, DY ;

Liu, L ;

Chen, HJ ;

Sawamura, T ;

Mehta, JL .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2003, 23 (05) :816-821

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