Growth of organic solvent-tolerant Pseudomonas aeruginosa LST-03 in the presence of various organic solvents and production of lipolytic enzyme in the presence of cyclohexane

Pseudomonas aeruginosa LST-03 grew in a liquid medium in the presence of organic solvents of which the log P value was equal to or greater than 2.4. Its growth in the presence of organic solvent was followed quantitatively by measuring the dry cell mass. Production of the lipolytic enzyme of P. aeruginosa LST-03 cultivated in the presence of cyclohexane was also investigated. Although this strain can grow in the presence of cyclohexane under conditions in which the supply of oxygen is limited, aeration is required for production of lipolytic enzyme. The Lipolytic activity of the supernatant of the culture in the presence of cyclohexane was stabler than that in the absence of organic solvent. (C) 1999 Elsevier Science S.A. All rights reserved.