Mode of action of acetylesterases associated with endoglucanases towards water-soluble and -insoluble cellulose acetates

被引：13

作者：

Altaner, C ^{[1
]}

Saake, B ^{[1
]}

Puls, J ^{[1
]}

机构：

[1] Fed Res Ctr Forestry & Forest Prod, Inst Wood Chem & Chem Technol Wood, D-21031 Hamburg, Germany

来源：

CELLULOSE | 2001年 / 8卷 / 04期

关键词：

acetyl esterase; cellulose acetate; endoglucanase; regioselective deacetylation;

D O I：

10.1023/A:1015164311521

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

A screening of commercial enzyme preparations for the capability of cellulose acetate (CA) deacetylation revealed that such enzyme activity is more common than could be anticipated. Enzyme-aided deacetylation of cellulose acetate was clearly a function of the degree of substitution (DS). Cellulose acetates up to a DS of 1.4 were deacetylated by a large number of enzyme mixes. Interestingly, none of the investigated enzyme preparations had the capability for complete cellulose acetate deacetylation. Definitely most hydrolase preparations investigated in our study deacetylated cellulose acetate with no preference for a certain position, as could be confirmed by NMR spectroscopy. However, one acetyl esterase had a clear preference for the C2- and C3-positions, leaving the acetyl substituents at the C6-position intact.

引用

页码：259 / 265

页数：7

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