Synergistic transactivation of HNF-1 alpha, HNF-3, and NF-I contributes to the activation of the liver-specific protein C gene

被引:19
作者
Tsay, W
Lee, YM
Lee, SC
Shen, MC
Chen, PJ
机构
[1] NATL TAIWAN UNIV, COLL MED, CLIN LAB, TAIPEI 10018, TAIWAN
[2] NATL TAIWAN UNIV, COLL MED, INST MOL MED, TAIPEI 10018, TAIWAN
[3] NATL TAIWAN UNIV, COLL MED, INST CLIN MED, TAIPEI 10018, TAIWAN
[4] ACAD SINICA, INST BIOL CHEM, TAIPEI, TAIWAN
关键词
D O I
10.1089/dna.1997.16.569
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously characterized the functional cis elements of the protein C promoter. One hepatocyte nuclear factor-1 (HNF-1) site, three HNF-3 sites, and at least two NF-I sites have been identified within the 140-bp basal transcriptional unit of this promoter, Here we present evidence that either HNF-1 alpha or HNF-3 can cooperate with each other in binding to their cis elements, The results from the co-transfection assays in HeLa cells showed a novel synergistic transactivation between HNF-1 alpha and HNF-3. Our data further indicate that the unique overlapping of the HNF-3 sites, the specific spatial relationship of the sites, and the co-activator C/EBP all contributed to the synergistic interaction, Although NF-I itself has a weak transactivating effect, it apparently coordinates the transactivation complex formation, NF-I can synergistically enhance the transactivation of HNF-1 alpha or HNF-3. Taken together, the combinatorial interplay of HNF-1 alpha, HNF-3, and NF-I make a significant contribution to the activation of the liver-specific protein C gene.
引用
收藏
页码:569 / 577
页数:9
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