Facile method to detect oligonucleotides with functionalized polydiacetylene vesicles

被引：31

作者：

Wang, CG ^{[1
]}

Ma, ZF ^{[1
]}

Su, ZM ^{[1
]}

机构：

[1] NE Normal Univ, Dept Chem, Changchun 130024, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2006年 / 113卷 / 01期

基金：

中国国家自然科学基金;

关键词：

polydiacetylene; vesicle sensor; oligonucleotides; dimyristoylphosphatidylcholine;

D O I：

10.1016/j.snb.2005.03.106

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Polydiacetylene vesicles were used to detect oligonucleotides in the present paper. For this system. probe-oligonucleotide-functionalized polydiacetylene vesicles were used as indicators of color transition and amplification tags at the same time in the detection of oligonucleotides. The method described is simple, rapid, and suitable for detection of oligonucleotides by naked eye, The influences of dimyristoylphosphatidylcholine (DMPC) in the color changeable polydiacetylene vesicles on the detection of oligonucleotides were also studied. The experimental results demonstrated that DMPC can effectively adjust the mechanical stress of polydiacetylenic structure and the content of DMPC in polydiacetylene structure is a crucial factor for a more sensitive polydiacetylene-based sensor. The maximum colorimetric response for the detection of oligonucleotides was obtained for the polydiacetylene vesicle composed of TCDA/DMPC/probe-DNA with molar ratio of 0.60:0.39:0.01. Both cases for too much or for too less DMPC in polydiacetylene vesicles are not benefit to the detection for this system. The TEM images further confirmed the results obtained. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：510 / 515

页数：6

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