Comparison of direct inoculation and copan transport systems for isolation of Neisseria gonorrhoeae from endocervical specimens

被引:14
作者
Olsen, CC
Schwebke, JR
Benjamin, WH
Beverly, A
Waites, KB
机构
[1] Univ Alabama, Dept Pathol, Birmingham, AL 35233 USA
[2] Univ Alabama, Dept Med, Birmingham, AL 35233 USA
关键词
D O I
10.1128/JCM.37.11.3583-3585.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two commercial swab transport systems, Copan Amies gel agar with and without charcoal (Copan Diagnostics, Corona, Calif), were compared to direct inoculation onto modified Thayer-Martin medium for detection of Neisseria gonorrhoeae in 1,490 endocervical specimens obtained from women attending a sexually transmitted disease clinic. Copan swabs were held in the transport system for 24 h at room temperature prior to inoculation onto modified Thayer-Martin medium. All cultures were incubated at 35 degrees C in 5% CO2, and bacteria were identified on the basis of Gram stain, oxidase, and biochemical reactions. Copan Amies gel agar transport system without charcoal detected 77 of 81 (95%) direct inoculation culture-positive specimens, and Copan Amies gel agar transport system with charcoal detected 53 of 56 (95%) directly inoculated culture-positive specimens. Copan Amies gel agar without charcoal inoculated after 6 h supported growth of 56 (98%) positive cultures out of only 55 directly inoculated culture-positive specimens, This study demonstrates that Copan swabs represent a reasonable alternative; providing convenience, low cost, and ease of use while still maintaining a satisfactory recovery rate of N, gonorrhoeae from clinical specimens, if specimens can be inoculated onto selective media within a relatively short time period not involving overnight shipment.
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收藏
页码:3583 / 3585
页数:3
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