Physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus

被引：16

作者：

Chen, CJ

Leisy, DJ

Thiem, SM

机构：

[1] MICHIGAN STATE UNIV,DEPT ENTOMOL,E LANSING,MI 48824

[2] MICHIGAN STATE UNIV,GENET PROGRAM,E LANSING,MI 48824

[3] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824

[4] MICHIGAN STATE UNIV,PESTICIDE RES CTR,E LANSING,MI 48824

[5] OREGON STATE UNIV,DEPT AGR CHEM,CORVALLIS,OR 97331

来源：

JOURNAL OF GENERAL VIROLOGY | 1996年 / 77卷

关键词：

CELERY LOOPER; INFECTIVITY; BACULOVIRUS; REPLICATION; LEPIDOPTERA; APOPTOSIS; NOCTUIDAE; GENOME; CELLS; GENE;

D O I：

10.1099/0022-1317-77-2-167

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrosis virus (AfMNPV) DNA was constructed for restriction endonucleases EcoRI, HindIII, PstI and XhoI. The genome size was estimated to be 130 kbp. Ordering of the restriction fragments was accomplished by cross-blot hybridization, double digestion and DNA-DNA hybridization. The polyhedrin gene and homologous repeat (hr) regions were located by hybridization to the Autographa californica MNPV (AcMNPV) polyhedrin gene and hr4, respectively. Restriction pattern comparison and Southern blot analysis suggest that AfMNPV is closely related to AcMNPV.

引用

页码：167 / 171

页数：5

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