A di-acidic (DXE) code directs concentration of cargo during export from the endoplasmic reticulum

被引:131
作者
Nishimura, N
Bannykh, S
Slabough, S
Matteson, J
Altschuler, Y
Hahn, K
Balch, WE
机构
[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Dept Biol Mol, La Jolla, CA 92037 USA
[3] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA
关键词
D O I
10.1074/jbc.274.22.15937
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Efficient export of vesicular stomatitis virus glycoprotein (Vm-G), a type I transmembrane protein, from the endoplasmic reticulum requires a di-acidic code (DXE) located in the cytosolic carboxyl-terminal tail (Nishimura, N,, and Balch, W, E, (1997) Science 277, 558-558), Mutation of the DXE code by mutation to AXA did not prevent VSV-G recruitment to pre-budding complexes formed in the presence of the activated form of the Sari and the Sec23/24 complex, components of the COPII budding machinery. However, the signal was required at a subsequent concentration step preceding vesicle fission. By using green fluorescence protein-tagged VSV-G to image movement in a single cell, we found that VSV-G lacking the DXE code fails to be concentrated into COPII vesicles. As a result, the normal 5-10-fold increase in the steady-state concentration of VSV-G in downstream pre-Golgi intermediates and Golgi compartments was lost. These results demonstrate for the first time that inactivation of the DXE signal uncouples early cargo selection steps from concentration into COPII. vesicles. We propose that two sequential steps are required for efficient export from the endoplasmic reticulum.
引用
收藏
页码:15937 / 15946
页数:10
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