Resistance of degraded hair shafts to contaminant DNA

被引：48

作者：

Gilbert, MTP

Menez, L

Janaway, RC

Tobin, DJ

Cooper, A

Wilson, AS

机构：

[1] Univ Oxford, Dept Zool, Henry Wellcome Ancient Biomol Ctr, Oxford OX1 3PS, England

[2] Bournemouth Univ, Sch Conservat Sci, Poole BH12 5BB, Dorset, England

[3] Univ Bradford, Dept Archaeol Sci, Bradford BD7 1DP, W Yorkshire, England

[4] Univ Bradford, Dept Biomed Sci, Bradford BD7 1DP, W Yorkshire, England

来源：

FORENSIC SCIENCE INTERNATIONAL | 2006年 / 156卷 / 2-3期

基金：

英国惠康基金;

关键词：

contamination; degradation; hair; mtDNA;

D O I：

10.1016/j.forsciint.2005.02.021

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

We have investigated the susceptibility of degraded human hair shaft samples to contamination by exogenous sources of DNA, including blood, saliva, skin cells, and purified DNA. The results indicate that on the whole hair shafts are either largely resistant to penetration by contaminant DNA. or extremely easy to successfully decontaminate. This pertains to samples that are both morphologically and biochemically degraded. We suggest that this resistance to the incorporation of contaminant DNA relates to the hydrophobic and impermeable nature of the keratin structures forming the hair shaft. Therefore, hair samples represent an important and underestimated source of DNA in both forensic and ancient DNA studies. (C) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：208 / 212

页数：5

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