Effects of quercetin studied in colonic HT-29/B6 cells and rat intestine in vitro

被引：40

作者：

Amasheh, Maren ^{[1
]}

Luettig, Julia ^{[1
]}

Amasheh, Salah ^{[2
]}

Zeitz, Martin ^{[1
]}

Fromm, Michael ^{[2
]}

Schulzke, Joerg-Dieter ^{[1
]}

机构：

[1] Charite, Dept Gastroenterol Infect Dis & Rheumatolo, Div Nutr Med, D-13353 Berlin, Germany

[2] Charite, Inst Clin Physiol, D-13353 Berlin, Germany

来源：

BARRIERS AND CHANNELS FORMED BY TIGHT JUNCTION PROTEINS II | 2012年 / 1258卷

关键词：

flavonoid; barrier function; tight junction; TNF-alpha; claudins; NECROSIS-FACTOR-ALPHA; EPITHELIAL BARRIER FUNCTION; TIGHT JUNCTIONS; ULCERATIVE-COLITIS; INTERFERON-GAMMA; CROHNS-DISEASE; CACO-2; CELLS; EXPRESSION; APOPTOSIS; INFLAMMATION;

D O I：

10.1111/j.1749-6632.2012.06609.x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to analyze the influence of quercetin on intestinal barrier function using the human colonic epithelial cell line HT-29/B6 and rat small and large intestine in vitro. Rat native ileum and late distal colon were incubated in Ussing chambers, and the total resistance (R-T) was measured, and expression of tight junction proteins was characterized in immunoblots. By simulating inflammatory conditions with TNF-alpha, we examined the barrier-preventive effects of quercetin. Incubation with TNF-alpha led to a decrease of R-T in HT-29/B6 cell monolayers, which could be partially inhibited by quercetin. In accordance with cell culture experiments, quercetin increased mucosal resistance of rat ileumand late distal colon. Thus, barrier disturbance in late distal colon specimens induced by TNF-alpha and IFN-gamma could be partially prevented by coincubation with quercetin. These findings demonstrate that quercetin enhances barrier function in rat small and large intestine and possesses protective effects on cytokine-induced barrier damage.

引用

页码：100 / 107

页数：8

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