Effects of Stress-Shielding on the Dynamic Viscoelasticity and Ordering of the Collagen Fibers in Rabbit Achilles Tendon

被引：18

作者：

Ikoma, Kazuya ^{[1
]}

Kido, Masamitsu ^{[1
]}

Nagae, Masateru ^{[1
]}

Ikeda, Takumi ^{[1
]}

Shirai, Toshiharu ^{[1
]}

Ueshima, Keiichiro ^{[1
]}

Arai, Yuji ^{[1
]}

Oda, Ryo ^{[1
]}

Fujiwara, Hiroyoshi ^{[1
]}

Kubo, Toshikazu ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Orthopaed, Kyoto, Japan

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2013年 / 31卷 / 11期

基金：

日本学术振兴会;

关键词：

Achilles tendon; stress shielding; dynamic viscoelasticity; proton double quantum filtered NMR; FILTERED NMR-SPECTROSCOPY; CONNECTIVE-TISSUE; BIOMECHANICAL PROPERTIES; IN-SITU; H-1; STRAIN; CRIMP; RELAXATION; MATURATION; MICROSCOPY;

D O I：

10.1002/jor.22424

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

We investigated the effects of stress-shielding on both viscoelastic properties and microstructure of collagen fibers in the Achilles tendon by proton double-quantum filtered (H-1-DQF) NMR spectroscopy. The right hind-limbs of 20 Japanese white rabbits were immobilized for 4 weeks in a cast with the ankle in plantarflexion. Dynamic viscoelasticity of the Achilles tendons was measured using a viscoelastic spectrometer. Proton DQF NMR signals were analyzed to determine the residual dipolar coupling of bound water molecules in the Achilles tendons. Both the dynamic storage modulus (E) and dynamic loss modulus (E) decreased significantly in the Achilles tendons of the stress-shielding group. The results of the H-1-DQF NMR examination demonstrated significantly reduced residual dipolar coupling in the Achilles tendons of this same group. The disorientation of collagen fibers by stress-shielding should contribute to degradation of the dynamic storage and loss moduli. The alterations of the collagen fiber orientation that contributed to the function of tendinous tissue can be evaluated by performing an analysis of H-1 DQF NMR spectroscopy. (c) 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31:1708-1712, 2013

引用

页码：1708 / 1712

页数：5

共 29 条

[1]

STORAGE OF ELASTIC STRAIN-ENERGY IN MUSCLE AND OTHER TISSUES [J].