Identification of a Novel Dihydrodaidzein Racemase Essential for Biosynthesis of Equol from Daidzein in Lactococcus sp Strain 20-92

被引：77

作者：

Shimada, Yoshikazu ^{[1
]}

Takahashi, Masayuki ^{[1
]}

Miyazawa, Norihiro ^{[2
]}

Abiru, Yasuhiro ^{[3
]}

Uchiyama, Shigeto ^{[3
]}

Hishigaki, Haretsugu ^{[1
]}

机构：

[1] Otsuka Pharmaceut Co Ltd, Inst Biomed Innovat, Tokushima 77101, Japan

[2] Otsuka Pharmaceut Co Ltd, Tokushima Res Inst, Drug Safety Res Ctr, Dept Drug Metab, Tokushima 77101, Japan

[3] Otsuka Pharmaceut Co Ltd, Saga Nutraceut Res Inst, Saga, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 14期

关键词：

HUMAN INTESTINAL BACTERIUM; ESTROGEN-RECEPTOR-ALPHA; PHYTOESTROGENS; ISOFLAVONES; METABOLISM; (3S)-EQUOL; ALIGNMENT; REDUCTASE; BETA;

D O I：

10.1128/AEM.00410-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Equol is metabolized from daidzein, a soy isoflavone, by the gut microflora. In this study, we identified a novel dihydrodaidzein racemase (L-DDRC) that is involved in equol biosynthesis in a lactic acid bacterium, Lactococcus sp. strain 20-92, and confirmed that histidine-tagged recombinant L-DDRC (L-DDRC-His) was able to convert both the (R)- and (S)-enantiomers of dihydro-daidzein to the racemate. Moreover, we showed that recombinant L-DDRC-His was essential for in vitro equol production from daidzein by a recombinant enzyme mixture and that efficient in vitro equol production from daidzein was possible using at least four enzymes, including L-DDRC. We also proposed a model of the metabolic pathway from daidzein to equol in Lactococcus strain 20-92.

引用

页码：4902 / 4907

页数：6

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