PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C-elegans

被引：485

作者：

Batista, Pedro J. ^{[3
,5
]}

Ruby, J. Graham ^{[1
,2
,6
]}

Claycomb, Julie M. ^{[3
]}

Chiang, Rosaria ^{[1
,2
,6
]}

Fahlgren, Noah ^{[7
,8
]}

Kasschau, Kristin D. ^{[7
,8
]}

Chaves, Daniel A. ^{[3
]}

Gu, Weifeng ^{[3
]}

Vasale, Jessica J. ^{[3
]}

Duan, Shenghua ^{[3
]}

Conte, Darryl, Jr. ^{[3
]}

Luo, Shujun ^{[9
]}

Schroth, Gary P. ^{[9
]}

Carrington, James C. ^{[7
,8
]}

Bartel, David P. ^{[1
,2
,6
]}

Mello, Craig C. ^{[3
,4
]}

机构：

[1] MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA

[2] MIT, Dept Biol, Cambridge, MA 02139 USA

[3] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

[4] Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01605 USA

[5] Gulbenkian PhD Programme Biomed, P-2780156 Oeiras, Portugal

[6] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[7] Oregon State Univ, Ctr Gene Res & Biotechnol, Corvallis, OR 97331 USA

[8] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA

[9] Illumina Inc, Hayward, CA 94545 USA

来源：

MOLECULAR CELL | 2008年 / 31卷 / 01期

关键词：

D O I：

10.1016/j.molcel.2008.06.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility.

引用

页码：67 / 78

页数：12

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