Prevalence of human gammaretrovirus XMRV in sporadic prostate cancer

被引:129
作者
Fischera, Nicole [1 ]
Hellwinkel, Olaf [2 ,3 ]
Schulz, Claudia [1 ]
Chun, Felix K. H. [2 ,3 ]
Huland, Hartwig [2 ,3 ]
Aepfelbacher, Martin [1 ]
Schlomm, Thorsten [2 ,3 ]
机构
[1] Univ Med Ctr Eppendorf, Inst Med Microbiol & Virol, D-20246 Hamburg, Germany
[2] Univ Med Ctr Eppendorf, Martini Clin, Prostate Canc Ctr, D-20246 Hamburg, Germany
[3] Univ Med Ctr Eppendorf, Dept Urol, D-20246 Hamburg, Germany
关键词
XMRV; Non-familial prostate cancer; RNase L; Infection; RT-PCR;
D O I
10.1016/j.jcv.2008.04.016
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: We previously identified a novel exogenous gammaretrovirus (xenotropic murine leukemia virus-related gammaretrovirus (XMRV)) using a pan-viral microarray. XMRV is the first MLV-related virus found in human infection. Forty percent (8/20) of familial prostate cancer patients homozygous for a Mutation in RNase L(R462Q) were positive for XMRV, while the virus was rarely(1166) detected in familial prostate cancer patients heterozygous for R462Q or carrying the wild type allele. Objectives: To determine the presence of XMRV in non-familial prostate cancer samples. Study design: RNA from prostate tissue was analyzed for XMRV using nested RT-PCR. In all samples, RNase L (R462Q) genotyping was performed using an allele-specific PCR. Results: XMRV-specific sequences were detected in one of 105 tissue samples from non-familial prostate cancer patients and from one of 70 tissue samples from men without prostate cancer. The two XMRV-positive patients were wild type or heterozygous for the R462Q mutation and thus carried at least one fully functional RNase L allele. Conclusions: XMRV was rarely detected in non-familial prostate cancer samples from Northern European patients. The homozygous mutation R462Q(QQ) was significantly underrepresented (<6%) in this cohort when compared to other studies (11 -17%). (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:277 / 283
页数:7
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