Alcohol biosensor based on the immobilization of meldola blue and alcohol dehydrogenase into a carbon nanotube paste electrode

被引:32
作者
Antiochia, R [1 ]
Lavagnini, I [1 ]
机构
[1] Univ Padua, Dipartimento Sci Chim, I-35171 Padua, Italy
关键词
biosensor; ethanol; alcohol dehydrogenase; Meldola Blue; carbon nanotube paste electrode;
D O I
10.1080/00032710600713537
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The preparation and electrochemical characterization of a new carbon nanotube paste (CNTP) amperometric biosensor for ethanol is described. The biosensor is formed by a CNTP electrode modified with the phenoxazine compound Meldola Blue (MB) as mediator. It shows a good electrocatalytic activity towards NADH oxidation at potentials around 0.0 V vs. Ag/AgCl, which represents a strong diminution in the overpotential. The biosensor is based on the activity of a commercially available alcohol dehydrogenase enzyme (ADH) and is realized by co-immobilizing the enzyme ADH, the coenzyme NAD + , and the mediator into the paste. Analytical parameters such as enzyme, co-enzyme and mediator immobilization concentration, pH, temperature, and probe lifetime are studied and optimized. The biosensor yields a linear response to 1x10(-7) -4x10(-6) mol/L ethanol at an operating potential of +50 mV (vs. Ag/AgCl), where interfering reactions do not occur, with a detection limit of 1x10(-7) mol/L and a good reproducibility (RSD of 2.8%, n=6). Application of the biosensor to the determination of ethanol in alcoholic beverages was achieved successfully.
引用
收藏
页码:1643 / 1655
页数:13
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