Protective Effect of Tanshinone IIA Against Infarct Size and Increased HMGB1, NFκB, GFAP and Apoptosis Consequent to Transient Middle Cerebral Artery Occlusion

被引：84

作者：

Wang, Jian-Gang ^{[1
]}

Bondy, Stephen C. ^{[2
]}

Zhou, Li ^{[3
]}

Yang, Feng-Zhen ^{[4
]}

Ding, Zhi-Gang ^{[3
]}

Hu, Yu ^{[3
]}

Tian, Yun ^{[3
]}

Wen, Pu-Yuan ^{[5
]}

Luo, Hao ^{[4
]}

Wang, Fang ^{[4
]}

Li, Wen-Wen ^{[4
]}

Zhou, Jun ^{[3
]}

机构：

[1] Third Xiang Ya Hosp, Dept Neurol, Changsha 410013, Hunan, Peoples R China

[2] Univ Calif Irvine, Ctr Occupat & Environm Hlth, Dept Med, Irvine, CA 92697 USA

[3] Third Xiang Ya Hosp, Ctr Med Expt, Changsha 410013, Hunan, Peoples R China

[4] Xiang Ya Sch Med, Dept Clin Microbiol & Immunol, Changsha 410013, Hunan, Peoples R China

[5] Third Xiang Ya Hosp, Dept Cardiol, Changsha 410013, Hunan, Peoples R China

来源：

NEUROCHEMICAL RESEARCH | 2014年 / 39卷 / 02期

关键词：

Apoptosis; Inflammation; Middle cerebral artery occlusion; Tanshinone IIA; DENDRITIC CELLS; ISCHEMIA; ACTIVATION; STROKE; DEATH; RELEASE; NEURONS; PATHWAY; MODELS; MOUSE;

D O I：

10.1007/s11064-013-1221-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Acute inflammation plays an important role in brain damage following cerebral ischemia and reperfusion (I/R) injury. The present study employed a rat model of middle cerebral artery occlusion to explore the neuroprotective effects of tanshinone IIA (TSN), which is widely used in China for treating cerebrovascular and cardiovascular diseases. Rats were divided into a sham-operated group and I/R transiently occluded then reperfused groups. Some of the I/R animals were treated daily for 7 or 15 days with two different doses of TSN. After 15 days, triphenyl tetrazolium chloride staining revealed less unstained area indicating fewer lesions in the TSN-treated I/R group relative to the untreated corresponding I/R group. TSN treatment dramatically reduced infarct sizes and reduced content of high mobility group box 1 protein following I/R. Nuclear translocation of NF kappa B was also attenuated in I/R animals subsequently receiving TSN. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining revealed more apoptosis in the I/R model group and this was reduced in the I/R animals treated with TSN for 15 days. Thus, TSN mitigates the severity of damage effected by I/R.

引用

页码：295 / 304

页数：10

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