Chemical nanoprinting: a novel method for fabricating DNA microchips

被引：18

作者：

Kumar, Anil ^{[1
]}

Liang, Zicai ^{[1
]}

机构：

[1] Karolinska Inst, Ctr Genom Res, Genom Technol Unit, S-17177 Stockholm, Sweden

来源：

NUCLEIC ACIDS RESEARCH | 2001年 / 29卷 / 02期

关键词：

D O I：

10.1093/nar/29.2.e2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a novel cost-effective procedure, namely 'chemical nanoprinting', for oligonucleotide or cDNA chips manufacture. In this thermo-controlled process, the oligonucleotides, covalently attached to a highly loaded 'master-chip' through disulfide bonds, are chemically transferred to the acrylamide layer mounted on a 'print-chip'. It is demonstrated here that multiple identical print-chips can be produced from a single master-chip. This duplication process is a few hundreds of times faster than any existing methods and the speed of process and cost incurred are independent of the scale of the DNA chips.

引用

页数：4

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