Leuconostoc mesenteroides glucansucrase synthesis of flavonoid glucosides by acceptor reactions in aqueous-organic solvents

被引：80

作者：

Bertrand, A

Morel, S

Lefoulon, F

Rolland, Y

Monsan, P

Remaud-Simeon, M

机构：

[1] Inst Natl Sci Appl, Lab Biotechnol Bioprocedes, CNRS, UMR 5504,INRA,UMR 792,DGBA, F-31077 Toulouse 04, France

[2] Technol Servier, F-45000 Orleans, France

[3] Labs Servier, F-92200 Neuilly Sur Seine, France

来源：

CARBOHYDRATE RESEARCH | 2006年 / 341卷 / 07期

关键词：

flavonoid; glucosylation; luteolin; glucansucrase; Leuconostoc mesenteroides; acceptor reaction;

D O I：

10.1016/j.carres.2006.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymatic glucosylation of luteolin was attempted using two glucansucrases: the dextransucrase from Leuconostoc mesenteroides NRRL B-512F and the alternansucrase from L. mesenteroides NRRL B-23192. Reactions were carried out in aqueous-organic solvents to improve luteolin solubility. A molar conversion of 44% was achieved after 24 h of reaction catalysed by dextransucrase from L. mesenteroides NRRL B-512F in a Mixture of acetate buffer (70%)/bis(2-methoxyethyl) ether (30%). Two products were characterised by nuclear magnetic resonance (NMR) spectroscopy: luteolin-3'-O-alpha-D-glucopyranoside and lutcolin-4'-O-alpha-D-glucopyranoside. In the presence of alternansucrase from L. mesenteroides NRRL B-23192, three additional products were obtained with a luteolin conversion of 8%. Both enzymes were also able to glucosylate quercetin and myricetin with conversion of 4% and 49%, respectively. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：855 / 863

页数：9

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