Dynamic 18F-fluoride small animal PET to noninvasively assess renal function in rats

被引：31

作者：

Schnoeckel, Uta ^{[1
]}

Reuter, Stefan ^{[2
]}

Stegger, Lars ^{[1
]}

Schlatter, Eberhard ^{[2
]}

Schaefers, Klaus P. ^{[1
]}

Hermann, Sven ^{[1
]}

Schober, Otmar ^{[1
]}

Gabrieels, Gert ^{[2
]}

Schaefers, Michael ^{[1
]}

机构：

[1] Nukl Med Klin & Poliklin, D-48149 Munster, Germany

[2] Univ Klinikum Munster, Med Klin & Poliklin D, Munster, Germany

来源：

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING | 2008年 / 35卷 / 12期

关键词：

Renal disease; Renography quantitative; Small animal PET; Small animal imaging modeling PET/SPECT quantification; Small animal PET molecular imaging PET instrumentation tomographic reconstruction;

D O I：

10.1007/s00259-008-0878-y

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Purpose Renal function can be quantified by both laboratory and scintigraphic methods. In the case of small animal diagnostics, scintigraphic image-based methods are ideal since they can assess split renal function, work non-invasively, and can be repeated. The aim of this study is to validate a F-18-PET-based method to quantify renal function in rats. Materials and methods Fluoride clearance was calculated from a dynamic whole body listmode acquisition of 60 min length in a small animal PET scanner following an i.v. injection of 15 MBq F-18-fluoride. Volumes of interest (VOIs) were placed in the left ventricle and the bladder as well as traced around the kidney contours. The respective time-activity curves (TAC) were calculated. The renal F-18-clearance was calculated by the ratio of the total renal excreted activity (bladder VOI) and the integral of the blood TAC. PET-derived renal function was validated by intraindividual measurements of creatinine clearance (n = 23), urea clearance (n = 23), and tubular excretion rate (TER-MAG3). The split renal function was derived from the injection of the clinically available radionuclide Tc-99m-mercaptotriglycine by blood sampling and planar renography (n = 8). Results In all animals studied, PET revealed high-quality TACs. PET-derived renal fluoride clearance was linearly correlated with intraindividual laboratory measures (PET vs. creatinine: r = 0.78; PET vs. urea: r = 0.73; PET vs. TER-MAG3: r = 0.73). Split function was comparable (F-18-PET vs. MAG3-renography: r = 0.98). PET-derived measures were highly reproducible. Conclusions F-18-PET is able to noninvasively assess renal function in rats and provides a significant potential for serial studies in different experimental scenarios.

引用

页码：2267 / 2274

页数：8

共 26 条

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