DNA polymerase ε catalytic domains are dispensable for DNA replication, DNA repair, and cell viability

被引：223

作者：

Kesti, T

Flick, K

Keränen, S

Syväoja, JE

Wittenberg, C

机构：

[1] Scripps Res Inst, Dept Biol Mol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[3] VTT, Biotechnol & Food Res, FIN-02044 Espoo, Finland

[4] Univ Oulu, Bioctr, FIN-90571 Oulu, Finland

[5] Univ Oulu, Dept Biochem, FIN-90571 Oulu, Finland

来源：

MOLECULAR CELL | 1999年 / 3卷 / 05期

关键词：

D O I：

10.1016/S1097-2765(00)80361-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA polymerase epsilon (Pol epsilon) is believed to play an essential catalytic role during eukaryotic DNA replication and is thought to participate in recombination and DNA repair. That pol epsilon is essential for progression through S phase and for viability in budding and fission yeasts is a central element of support for that view. We show that the amino-terminal portion of budding yeast Pol epsilon (Pol2) containing all known DNA polymerase and exonuclease motifs is dispensable for DNA replication, DNA repair, and viability. However, the carboxy-terminal portion of Pol2 is both necessary and sufficient for viability. Finally, the viability of cells lacking Pol2 catalytic function does not require intact DNA replication or damage checkpoints.

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页码：679 / 685

页数：7

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