High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array

被引：294

作者：

Spurgeon, Sandra L. ^{[1
]}

Jones, Robert C. ^{[1
]}

Ramakrishnan, Ramesh ^{[1
]}

机构：

[1] Fluidigm Corp, San Francisco, CA USA

来源：

PLOS ONE | 2008年 / 3卷 / 02期

关键词：

D O I：

10.1371/journal.pone.0001662

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.

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页数：7

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