Long-term stability of 8-methoxypsoralen in ointments for topical PUVA therapy ('cream-PUVBA')

被引:7
作者
Martens-Lobenhoffer, J
Rinke, M
Lösche, D
Gollnick, H
机构
[1] Otto von Guericke Univ, Fac Med, Inst Clin Pharmacol, D-39120 Magdeburg, Germany
[2] Otto von Guericke Univ, Fac Med, Dept Dermatol & Venerol, D-39120 Magdeburg, Germany
[3] Otto von Guericke Univ, Fac Med, Cent Pharm, D-39120 Magdeburg, Germany
来源
SKIN PHARMACOLOGY AND APPLIED SKIN PHYSIOLOGY | 1999年 / 12卷 / 05期
关键词
PUVA therapy; 8-methoxypsoralen; stability; psoriasis;
D O I
10.1159/000066251
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
8-Methoxypsoralen (8-MOP) is an established photochemotherapeutic agent for PUVA therapy. Recently, a so-called 'cream-PUVA' modality was introduced into therapy of psoriasis and other dermatoses. Little is known, however, about the stability of 8-MOP in ointments used for the topical application of this compound. Therefore, we investigated the long-term stability of 8-MOP in three different ointments, Unguentum Gordes(TM), Cold Cream Naturel(TM) and a water containing gel on the basis of Carbopol 940. All three ointments were prepared with 8-MOP concentrations of 0.05 and 0.005%, and stored over 12 weeks at room temperature (19-20 degrees C) and at 5 degrees C. 8-MOP concentrations were measured at days 1. 8, 15, 29, 57 and 88 after preparation by thin-layer chromatography (TLC). The ointments were dissolved in an organic solvent, 10 mu l were transferred onto the TLC plate and the chromatograms were developed first in toluene and then in toluene/ethyl acetate 2:1 v/v to resolve 8-MOP from the ointment constituents. the peak heights of 8-MOP were used for quantitation. The intraday variabilities are <3% for Unguentum Cordes and Cold Cream Natural and <6% for the Carbopol 940 gel. The interday variabilities were <6.3% in all cases. In Unguentum Cordes and Cold Cream Naturel the concentrations of 8-MOP remain stable, but in Unguentum Cordes the emulsion began to break up after 8 weeks. In the Carbopol gel, only about 40% of the nominal concentrations of 8-MOP were found and they decrease significantly at storage at 5 degrees C. We conclude that the Carbopol gel seems to be unsuitable for PUVA therapy, whereas Cold Cream Naturel shows the best results.
引用
收藏
页码:266 / 270
页数:5
相关论文
共 8 条
[1]  
DERIE MA, 1995, BRIT J DERMATOL, V132, P964
[2]   PUVA THERAPY FOR PSORIASIS - COMPARISON OF ORAL AND BATH-WATER DELIVERY OF 8-METHOXYPSORALEN [J].
LOWE, NJ ;
WEINGARTEN, D ;
BOURGET, T ;
MOY, LS .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1986, 14 (05) :754-760
[3]   Rapid decrease of phototoxicity after PUVA bath therapy with 8-methoxypsoralen [J].
Neumann, NJ ;
Ruzicka, T ;
Lehmann, P ;
Kerscher, M .
ARCHIVES OF DERMATOLOGY, 1996, 132 (11) :1394-1394
[4]   THE EVOLUTION OF PHOTOCHEMOTHERAPY WITH PSORALENS AND UVA (PUVA) - 2000 BC TO 1992 AD [J].
PATHAK, MA ;
FITZPATRICK, TB .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 14 (1-2) :3-22
[5]   Cream-PUVA-phototherapy [J].
Stege, H ;
Berneburg, M ;
Ruzicka, T ;
Krutmann, J .
HAUTARZT, 1997, 48 (02) :89-93
[6]   Malignant melanoma in patients treated for psoriasis with methoxsalen (psoralen) and ultraviolet A radiation (PUVA) [J].
Stern, RS ;
Nichols, KT ;
Vakeva, LH .
NEW ENGLAND JOURNAL OF MEDICINE, 1997, 336 (15) :1041-1045
[7]   MINIMIZING THE RISKS OF PUVA TREATMENT [J].
VANPRAAG, MCG ;
TSENG, LNL ;
MOMMAAS, AM ;
BOOM, BW ;
VERMEER, BJ .
DRUG SAFETY, 1993, 8 (05) :340-349
[8]  
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