Modulation of the light response by cAMP in Drosophila photoreceptors

被引:58
作者
Chyb, S
Hevers, W
Forte, M
Wolfgang, WJ
Selinger, Z
Hardie, RC
机构
[1] Univ Cambridge, Dept Anat, Cambridge CB2 3DY, England
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 3DY, England
[3] Univ Cambridge, Dept Zool, Cambridge CB2 3DY, England
[4] Oregon Hlth & Sci Univ, Vollum Inst, Portland, OR 97201 USA
[5] Hebrew Univ Jerusalem, Inst Life Sci, Dept Biol Chem, IL-91904 Jerusalem, Israel
关键词
phototransduction; neuromodulation; TRP; photoreceptor; dopamine; calcium; cross-talk; adenylyl cyclase; phospholipase C;
D O I
10.1523/JNEUROSCI.19-20-08799.1999
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Phototransduction in Drosophila is mediated by a G-protein-coupled phospholipase C transduction cascade in which each absorbed photon generates a discrete electrical event, the quantum bump. In whole-cell voltage-clamp recordings, cAMP, as well as its nonhydrolyzable and membrane-permeant analogs 8-bromo-cAMP (8-Br-cAMP) and dibutyryl-cAMP, slowed down the macroscopic light response by increasing quantum bump latency, without changes in bump amplitude or duration. In contrast, cGMP or 8-Br-cGMP had no effect on light response amplitude or kinetics. None of the cyclic nucleotides activated any channels in the plasma membrane. The effects of cAMP were mimicked by application of the non-specific phosphodiesterase inhibitor IBMX and the adenylyl cyclase activator forskolin; zaprinast, a specific cGMP-phosphodiesterase inhibitor, was ineffective. Bump latency was also increased by targeted expression of either an activated Gs a subunit, which increased endogenous adenylyl cyclase activity, or an activated catalytic protein kinase A (PKA) subunit. The action of IBMX was blocked by pretreatment with the PKA inhibitor H-89. The effects of cAMP were abolished in mutants of the ninaC gene, suggesting this nonconventional myosin as a possible target for PKA-mediated phosphorylation. Dopamine (10 mu M) and octopamine (100 mu M) mimicked the effects of cAMP. These results indicate the existence of a G-protein-coupled adenylyl cyclase pathway in Drosophila photoreceptors, which modulates the phospholipase C-based phototransduction cascade.
引用
收藏
页码:8799 / 8807
页数:9
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