AFLPs mark different genomic regions compared with RFLPs: a case study in tomato

被引：17

作者：

Bonnema, G ^{[1
]}

Berg, P ^{[1
]}

Lindhout, P ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands

来源：

GENOME | 2002年 / 45卷 / 02期

关键词：

introgression lines; tomato; marker distribution; AFLP; RFLP; genetic linkage;

D O I：

10.1139/G01-145

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A set of three tomato chromosome 7 introgression lines (ILs) containing overlapping segments of Lycopersicon pennellii DNA was screened with a set of 10 EcoRI-MseI and 10 PstI-MseI AFLP primer combinations. A large number of markers were identified that mapped to one of the four regions of chromosome 7 defined by the set of three ILs. Because many of the identified markers have known map positions in three tomato reference maps, their location on the tomato genome could be verified. It was demonstrated that the three chromosome 7 ILs carried a chromosome 10 region harbouring a cluster of six AFLP markers that had not been detected before using RFLPs. The causes and implications of this observation are discussed.

引用

页码：217 / 221

页数：5

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