Surface plasmon resonance immunosensor for the detection of Salmonella typhi antibodies in buffer and patient serum

被引：30

作者：

Gupta, Garima ^{[1
]}

Sharma, P. K. ^{[1
]}

Sikarwar, B. ^{[1
]}

Merwyn, S. ^{[1
]}

Kaushik, S. ^{[2
]}

Boopathi, M. ^{[1
]}

Agarwal, G. S. ^{[1
]}

Singh, Beer ^{[1
]}

机构：

[1] Def Res & Dev Estab, DRDO, Gwalior 474002, India

[2] ITM Universe, Gwalior 474001, India

来源：

BIOSENSORS & BIOELECTRONICS | 2012年 / 36卷 / 01期

关键词：

Surface plasmon resonance; Salmonella typhi; Immunosensor; Impedance; r-Flagellin protein; STAPHYLOCOCCAL-ENTEROTOXIN-B; BIOSPECIFIC INTERACTION ANALYSIS; SPR-BASED IMMUNOSENSOR; FEVER; RECOGNITION; ADSORPTION; MONOLAYER; DIAGNOSIS; POLYMER; ANTIGEN;

D O I：

10.1016/j.bios.2012.03.046

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Surface plasmon resonance (SPR) immunosensor using 4-mercaptobenzoic acid (4-MBA) modified gold SPR chip was developed first time for the detection of flagellin specific antibodies of Salmonella typhi (S. typhi). Flagellin protein of S. typhi was prepared by recombinant DNA technology. The modification of gold chip with 4-MBA was in-situ characterized by SPR and electrochemical impedance spectroscopy. By using kinetic evaluation software, K-D and B-max values were calculated and found to be 26.3 fM and 62.04 m degrees, respectively, for the immobilized monoclonal antibody (Moab) of recombinant flagellin (r-fla) protein of S. typhi (r-fla S. typhi). In addition, thermodynamic parameters such as Delta G, Delta H and Delta S were determined first time for r-fla S. typhi and Moab of r-fla S. typhi interactions and the values revealed the interaction between r-fla S. typhi and Moab of r-fla S. typhi as spontaneous, endothermic and entropy driven one. Moreover, healthy human serum samples and patient sera (Widal positive and Widal negative) were subjected to SPR analysis. The present SPR based approach provides an alternative way for S. typhi detection in less than 10 min. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：95 / 102

页数：8

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