The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells

被引:135
作者
Chang, YC [1 ]
Huang, FM [1 ]
Tai, KW [1 ]
Chou, MY [1 ]
机构
[1] Chung Shan Med & Dent Coll, Sch Dent, Taichung, Taiwan
来源
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS | 2001年 / 92卷 / 04期
关键词
D O I
10.1067/moe.2001.116812
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective. The objective of this study was to examine the effects of sodium hypochlorite (NaOCl) and chlorhexidine (CHx) on cultured human periodontal ligament (PDL) cells in vitro. Study design. The effects of irrigation solutions on human PDL cells were evaluated by propidium iodide fluorescence cytotoxicity assay, protein synthesis assay, and mitochondrial activity. Results. Both NaOCl and CHx were cytotoxic to human PDL cells in a concentration- and contact time-dependent manner. In addition, CHx inhibited protein synthesis in human PDL cells. Although NaOCl displayed cellular cytotoxicity, it showed no protein inhibition in the PDL cells. Furthermore, both NaOCl and CHx exhibited an inhibitory effect on mitochondrial activity on human PDL cells. Conclusions. This study suggests that these irrigation fluids may cause detrimental effects on vital tissues. Its clinical significance, however, needs to be evaluated further because concentration used, exposure time to the agent, and exposure surface area are important factors affecting the resulting effect.
引用
收藏
页码:446 / 450
页数:5
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