Expression of a viral protein by muscle cells in vivo induces protective cell-mediated immunity

被引：39

作者：

Ulmer, JB

Deck, RR

DeWitt, CM

Fu, TM

Donnelly, JJ

Caulfield, MJ

Liu, MA

机构：

来源：

VACCINE | 1997年 / 15卷 / 08期

关键词：

DNA vaccine; myocyte; antigen presentation; MHC Class I;

D O I：

10.1016/S0264-410X(96)00256-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Intramuscular injection of plasmid DNA expression vectors results in transfection of myocytes in situ. To determine whether expression of antigen by myocytes is sufficient to induce protective cell-mediated immunity, stably transfected myoblasts expressing influenza nucleoprotein (NP) were transplanted into mice. These animals produced high-titer anti-NP antibodies and MHC class I-restricted cytotoxic T lymphocytes, and were protected from a cross-strain lethal challenge with influenza A virus. Therefore, antigen expression by muscle cells in vivo is sufficient to confer protective cell-mediated immunity. (C) 1997 Elsevier Science Ltd.

引用

收藏

页码：839 / 841

页数：3

相关论文

共 11 条

[1] IMMUNIZATION WITH DNA [J].

DONNELLY, JJ ;

ULMER, JB ;

LIU, MA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 176 (02) :145-152

[2]

DONNELLY JJ, 1995, ANN NY ACAD SCI, V772, P4047

[3]

DONNELLY JJ, 1995, EXPERT OPIN THER PAT, V5, P211

[4] ROLE OF BONE-MARROW-DERIVED CELLS IN PRESENTING MHC CLASS I-RESTRICTED TUMOR-ANTIGENS [J].

HUANG, AYC ;

GOLUMBEK, P ;

AHMADZADEH, M ;

JAFFEE, E ;

PARDOLL, D ;

LEVITSKY, H .

SCIENCE, 1994, 264 (5161) :961-965

[5] EXPOSING THE IMMUNOLOGY OF NAKED DNA VACCINES [J].

PARDOLL, DM ;

BECKERLEG, AM .

IMMUNITY, 1995, 3 (02) :165-169

[6] B lymphocytes secrete antigen-presenting vesicles [J].

Raposo, G ;

Nijman, HW ;

Stoorvogel, W ;

Leijendekker, R ;

Harding, CV ;

Melief, CJM ;

Geuze, HJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (03) :1161-1172

[7] COSTIMULATION OF LYMPHOCYTES-T - THE ROLE OF CD28, CTLA-4, AND B7/BB1 IN INTERLEUKIN-2 PRODUCTION AND IMMUNOTHERAPY [J].

SCHWARTZ, RH .

CELL, 1992, 71 (07) :1065-1068

[8] A MECHANISM FOR THE SPECIFIC IMMUNOGENICITY OF HEAT-SHOCK PROTEIN-CHAPERONED PEPTIDES [J].

SUTO, R ;

SRIVASTAVA, PK .

SCIENCE, 1995, 269 (5230) :1585-1588

[9] HETEROLOGOUS PROTECTION AGAINST INFLUENZA BY INJECTION OF DNA ENCODING A VIRAL PROTEIN [J].

ULMER, JB ;

DONNELLY, JJ ;

PARKER, SE ;

RHODES, GH ;

FELGNER, PL ;

DWARKI, VJ ;

GROMKOWSKI, SH ;

DECK, RR ;

DEWITT, CM ;

FRIEDMAN, A ;

HAWE, LA ;

LEANDER, KR ;

MARTINEZ, D ;

PERRY, HC ;

SHIVER, JW ;

MONTGOMERY, DL ;

LIU, MA .

SCIENCE, 1993, 259 (5102) :1745-1749

[10] Generation of MHC class I-restricted cytotoxic T lymphocytes by expression of a viral protein in muscle cells: Antigen presentation by non-muscle cells [J].

Ulmer, JB ;

Deck, RR ;

Dewitt, CM ;

Donnelly, JJ ;

Liu, MA .

IMMUNOLOGY, 1996, 89 (01) :59-67