Resistance genes for rye stem rust (SrR) and barley powdery mildew (Mla) are located in syntenic regions on short arm of chromosome

被引：20

作者：

Mago, R ^{[1
]}

Spielmeyer, W ^{[1
]}

Lawrence, GJ ^{[1
]}

Ellis, JG ^{[1
]}

Pryor, AJ ^{[1
]}

机构：

[1] CSIRO Plant Ind, Canberra, ACT 2601, Australia

来源：

GENOME | 2004年 / 47卷 / 01期

关键词：

DRA-1; Gabo; 1BL.1RS; imperial rye; interstitial deletion; resistance gene analog (RGA);

D O I：

10.1139/G03-096

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genetic stocks were developed for the localization and eventual cloning of the stem rust resistance gene SrR that occurs in wheat lines carrying the 1RS translocation from Secale cereale 'Imperial' rye. We have used a mutation-based approach for molecular analysis of the SrR region in rye. Forty-one independent mutants resulting in loss of SrR resistance were isolated: many of these were deletions of various sizes that were used to locate SrR with respect to chromosome group S markers. The analysis of the mutants showed that markers about 1 Mb apart flanking the barley Mla locus also flank SrR. Additionally, three of the approximately 20 closely related sequences of Mla in rye are deleted in each of six interstitial deletion mutants of SrR. The results indicate that the SrR region in rye is syntenic to the Mla region in barley or that SrR is possibly orthologous to the Mla locus.

引用

页码：112 / 121

页数：10

共 25 条

[1] Molecular analysis of irradiation induced and spontaneous deletion mutants at a disease resistance locus in Lactuca sativa [J].