Stimulation of Liver Regeneration After Hepatectomy in Mice by Injection of Bone Marrow Mesenchymal Stem Cells via the Portal Vein

被引：45

作者：

Kaibori, M. ^{[1
]}

Adachi, Y. ^{[3
]}

Shimo, T. ^{[2
]}

Ishizaki, M. ^{[1
]}

Matsui, K. ^{[1
]}

Tanaka, Y. ^{[1
]}

Ohishi, M. ^{[1
]}

Araki, Y. ^{[1
]}

Okumura, T. ^{[1
]}

Nishizawa, M. ^{[4
]}

Kwon, A. H. ^{[1
]}

机构：

[1] Kansai Med Univ, Dept Surg, Hirakata, Osaka 5731191, Japan

[2] Kansai Med Univ, Dept Pediat, Hirakata, Osaka 5731191, Japan

[3] Toyooka Hosp, Div Clin Pathol, Toyooka, Hyogo, Japan

[4] Ritsumeikan Univ, Coll Life Sci, Dept Biomed Sci, Kyoto, Shiga, Japan

来源：

TRANSPLANTATION PROCEEDINGS | 2012年 / 44卷 / 04期

关键词：

RAT-LIVER; FUSION; HEPATOCYTES; THERAPY; DISEASE;

D O I：

10.1016/j.transproceed.2012.01.088

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Aim. To investigate whether mouse bone marrow mesenchymal stem cells (BMC) stimulate liver regeneration after partial hepatectomy. Methods. Isolated BMCs were purified by density gradient centrifugation. We performed a 70% hepatectomy in male BALB/c mice followed by injection of BMCs into the portal vein (PV-BMC group), or the tail vein (IV-BMC group), or of saline into the portal vein (control group). Results. The wet weight of the liver remnant increased significantly in the PV-BMC group at 3 and 5 days after hepatectomy compared with the IV-BMC and control groups. The Ki-67 labeling index revealed that the increase to result from stimulation of DNA synthesis. The constitutive interleulcin-6 and hepatocyte growth factor mRNAs in the remnant liver tended to increase in the PV-BMC group at 3 days after hepatectomy. Conclusions. These results demonstrated that BMC injection into the portal vein enhanced liver growth after partial hepatectomy in mice.

引用

页码：1107 / 1109

页数：3

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