Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein ICP8

被引：3

作者：

Spatz, M

Ali, SA

Auer, M

Graf, C

Eibl, MM

Steinkasserer, A

机构：

[1] Vet Med Univ Wien, Inst Bakteriol Mykol & Hyg, Biomed Forsch Gesell MbH, A-1210 Vienna, Austria

[2] Novartis Res Inst, Dept Immunol, A-1235 Vienna, Austria

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1999年 / 16卷 / 01期

关键词：

herpes simplex virus; recombinant protein; ICP8; circular dichroism; baculovirus expression system;

D O I：

10.1006/prep.1999.1046

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>5 mg/10(8) cells) of ICP8. The recombinant ICPS is fully functional and binds to single-stranded DNA. Secondary structure calculations from circular dichroism measurements indicate a content of 34.5% or-helix and 15.4% beta-sheet. This is the first structural report for ICP8 using CD analysis, which will be very useful for high-throughput assay development and mechanistic studies, (C) 1999 Academic Press.

引用

页码：40 / 46

页数：7

共 39 条

[1] HERPES-SIMPLEX VIRUS TYPE-1 ICP8 - HELIX-DESTABILIZING PROPERTIES [J].