Rational design of an optical sensing system for multivalent proteins

被引:20
作者
Song, XD [1 ]
Swanson, BI [1 ]
机构
[1] Univ Calif Los Alamos Natl Lab, Chem Sci & Technol Div, Los Alamos, NM 87545 USA
关键词
D O I
10.1021/la980758k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A generic design principle for detection of multivalent interactions is described. A phospholipid bilayer consisting of natural and pyrene-derivatized phosphatidylcholines is used as both a supporting biomimetic surface and part of a signal transduction element. The pyrene excimer formed in the surface can act as fluorescence donor, and DABCY/BODIPY-FL covalently attached to receptor (GM1) can act as acceptors. Aggregation of the acceptor-tagged receptors resulting from multivalent binding of CT induces a decrease in efficiency of fluorescence quenching of the pyrene excimer by DABCY or energy transfer from pyrene excimer to BODIPY-FL. In the case using fluorescent acceptors that can undergo distance-dependent fluorescence self-quenching capability make acceptor fluorescence go down even further by th binding. This scheme can achieve signal amplification and high surface density of the optical transduction elements, which, in return, require relatively small surface area.
引用
收藏
页码:4710 / 4712
页数:3
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