Involvement of Golgin-160 in cell surface transport of renal ROMK channel: Co-expression of Golgin-160 increases ROMK currents

被引:18
作者
Bundis, F
Neagoe, I
Schwappach, B
Steinmeyer, K
机构
[1] Sanofi Aventis Deutsch GmbH, TD Cardiovasc, D-65926 Frankfurt, Germany
[2] Heidelberg Univ, Ctr Mol Biol, D-6900 Heidelberg, Germany
关键词
ROMK; inward rectifier; Golgin-160; yeast two-hybrid; membrane trafficking; cell surface transport;
D O I
10.1159/000091454
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The weak inward rectifier potassium channel ROMK is important for water and salt reabsorption in the kidney. Here we identified Golgin-160 as a novel interacting partner of the ROMK channel. By using yeast two-hybrid assays and co-immunoprecipitations from transfected cells, we demonstrate that Golgin-160 associates with the ROMK C-terminus. Immunofluorescence microscopy confirmed that both proteins are co-localized in the Golgi region. The interaction was further confirmed by the enhancement of ROMK currents by the co-expressed Golgin-160 in Xenopus oocytes. The increase in ROMK current amplitude was due to an increase in cell surface density of ROMK protein. Golgin-160 also stimulated current amplitudes of the related Kir2.1, and of voltage-gated Kv1.5 and Kv4.3 channels, but not the current amplitude of co-expressed HERG channel. These results demonstrate that the Golgi-associated Golgin-160 recognizes the cytoplasmic C-terminus of ROMK, thereby facilitating the transport of ROMK to the cell surface. However, the stimulatory effect on the activity of more distantly-related potassium channels suggests a more general role of Golgin-160 in the trafficking of plasma membrane proteins. Copyright (c) 2006 S.Karger AG, Basel.
引用
收藏
页码:1 / 12
页数:12
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