Immunoassay based on monoclonal antibody for aflatoxin detection in poultry feed

被引：57

作者：

Rossi, Carolina Nachi

Takabayashi, Cassia Reika ^{[2
]}

Ono, Mario Augusto ^{[3
]}

Saito, Gervasio Hitoshi

Itano, Eiko Nakagawa ^{[3
]}

Kawamura, Osamu ^{[4
]}

Hirooka, Elisa Yoko ^{[2
]}

Sataque Ono, Elisabete Yurie ^{[1
]}

机构：

[1] Univ Estadual Londrina, Dept Biochem & Biotechnol, Ctr Exact Sci, BR-86051980 Londrina, Parana, Brazil

[2] Univ Estadual Londrina, Dept Food Sci & Technol, BR-86051980 Londrina, Parana, Brazil

[3] Univ Estadual Londrina, Dept Pathol Sci, BR-86051980 Londrina, Parana, Brazil

[4] Kagawa Univ, Fac Agr, Dept Biochem & Food Sci, Miki, Kagawa 7610765, Japan

来源：

FOOD CHEMISTRY | 2012年 / 132卷 / 04期

关键词：

Mycotoxins; Enzyme-linked immunosorbent assay (ELISA); Broiler feed; Laying hen feed; Validation; COMPETITIVE ELISA; MYCOTOXINS; PRODUCTS; STATE; GRAIN; CORN; HPLC; KIT;

D O I：

10.1016/j.foodchem.2011.12.067

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on an anti-aflatoxin B-1 monoclonal antibody was standardised and validated for aflatoxin screening in poultry feed samples and its performance was compared to high-performance liquid chromatography (HPLC). The ic-ELISA showed good linearity (r(2) = 0.994) and detection limits of 1.25 ng g(-1) for broiler feed and 1.41 ng g(-1) for laying hen feed. Mean aflatoxin recovery rates by ic-ELISA were 102% (laying hen feed) and 98% (broiler feed). Aflatoxins were detected in 88.2% of the 34 broiler feed samples by ic-ELISA and HPLC at means of 10.48 ng g(-1) and 8.41 ng g(-1), respectively, while 92% of laying hen feed samples (n = 36) showed aflatoxin contamination at means of 20.83 and 19.75 ng g(-1). The standardised ic-ELISA showed reliability and a high correlation with HPLC of 0.97 (broiler feed) and 0.98 (laying hen feed) indicating its potential for aflatoxin screening in poultry feed samples. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：2211 / 2216

页数：6

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