Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase):: The key enzyme in U-insertion/deletion RNA editing

被引：110

作者：

Aphasizhev, R

Sbicego, S

Peris, M

Jang, SH

Aphasizheva, I

Simpson, AM

Rivlin, A

Simpson, L ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA

[3] Taegu Univ, Dept Mol Biol, Kyung San 712714, Kyung Pook, South Korea

[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[5] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

CELL | 2002年 / 108卷 / 05期

关键词：

D O I：

10.1016/S0092-8674(02)00647-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg2+. TUTase is present in vivo in at least two stable configurations: one contains a similar to500 kDa TUTase oligomer and the other a similar to700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.

引用

页码：637 / 648

页数：12

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