High-speed, two-photon scanning microscope

被引：132

作者：

Kim, KH ^{[1
]}

Buehler, C ^{[1
]}

So, PTC ^{[1
]}

机构：

[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

来源：

APPLIED OPTICS | 1999年 / 38卷 / 28期

关键词：

D O I：

10.1364/AO.38.006004

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed a high-speed two-photon microscope with submicrometer resolution in real time. The imaging speed improvement of this system is obtained by the use of a high-speed polygonal mirror scanner. The maximum achievable scanning rate is 40 mu s/line, which is approximately 100 times faster than conventional scanning microscopes. High-resolution fluorescence images were recorded in real time by an intensified CCD camera. Using this instrument, we have resolved cellular architecture in three dimensions and have monitored the movements of protozoas. More important, photodamage to biological specimens during video-rate imaging can be minimized with two-photon excitation as compared with other one-photon modalities. (C) 1999 Optical Society of America.

引用

页码：6004 / 6009

页数：6

共 11 条

[1]

Bennett BD, 1996, J BIOL CHEM, V271, P3647

[2] Multifocal multiphoton microscopy [J].