Study of the Association Between Polymorphisms of the COL1A1 Gene and HBV-Related Liver Cirrhosis in Chinese Patients

被引：13

作者：

Zhao, Yun-Peng ^{[1
]}

Wang, Hao ^{[2
]}

Fang, Meng ^{[1
]}

Ji, Qiang ^{[1
]}

Yang, Zai-Xing ^{[2
]}

Gao, Chun-Fang ^{[1
]}

机构：

[1] Second Mil Med Univ, Eastern Hepatobiliary Hosp, Dept Lab Med, Shanghai, Peoples R China

[2] Second Mil Med Univ, Changzheng Hosp, Dept Lab Med, Shanghai, Peoples R China

来源：

DIGESTIVE DISEASES AND SCIENCES | 2009年 / 54卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Cirrhosis; Fibrosis; Collagen; Polymorphism; Promoter; HEPATIC STELLATE CELLS; BONE-MINERAL DENSITY; ALPHA-1(I) PROCOLLAGEN GENE; FINGER TRANSCRIPTION FACTOR; I COLLAGEN GENES; INTERFERON-GAMMA; EXPRESSION; PROMOTER; FIBROSIS; FIBROBLASTS;

D O I：

10.1007/s10620-008-0340-9

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

To investigate the association between polymorphisms of the COL1A1 gene and liver cirrhosis. A total of 111 liver cirrhotic patients and 95 matched controls were recruited. Polymorphisms -1997T > G, -1663 ins/del T and -1363C > G of the COL1A1 gene were detected by direct sequencing. The activities of the putative promoters containing these polymorphisms were analyzed by means of the reporter gene system. No polymorphism at -1663 ins/del T was observed in any subject. Linkage disequilibrium was shown between -1997T > G and -1363C > G. The frequency of haplotype -1997T/-1363C was significantly higher in patients than that in controls. The putative promoters containing -1997T/-1363C resulted in higher reporter gene activity in LX-2. Strong transcriptional inhibition by IFN gamma was shown in both cells. The T allele at -1997 of COL1A1 is crucial to the increased transcriptional activity. COL1A1 gene polymorphism might be associated with liver fibrogenesis.

引用

页码：369 / 376

页数：8

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