Bursting the bubble on bacterial biofilms: a flow cell methodology

被引：78

作者：

Crusz, Shanika A. ^{[1
]}

Popat, Roman ^{[1
]}

Rybtke, Morten Theil ^{[2
]}

Camara, Miguel ^{[1
]}

Givskov, Michael ^{[2
,3
]}

Tolker-Nielsen, Tim ^{[2
]}

Diggle, Stephen P. ^{[1
]}

Williams, Paul ^{[1
]}

机构：

[1] Univ Nottingham, Ctr Biomol Sci, Sch Mol Med Sci, Nottingham NG7 2RD, England

[2] Univ Copenhagen, Dept Int Hlth Immunol & Microbiol, DK-2200 Copenhagen N, Denmark

[3] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore, Singapore

来源：

BIOFOULING | 2012年 / 28卷 / 08期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

biofilm; flow cell; bubble trap; Pseudomonas aeruginosa; method; protocol; QUANTIFICATION;

D O I：

10.1080/08927014.2012.716044

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.

引用

页码：835 / 842

页数：8

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