UVA radiation impairs phenotypic and functional maturation of human dermal dendritic cells

被引:24
作者
Furio, L [1 ]
Berthier-Vergnes, O [1 ]
Ducarre, B [1 ]
Schmitt, D [1 ]
Peguet-Navarro, J [1 ]
机构
[1] Univ Lyon 1, Dermatol Clin, Hop Edouard Herriot, F-69437 Lyon, France
关键词
dendritic cells; dermis; immune suppression; UVA;
D O I
10.1111/j.0022-202X.2005.23904.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
There is now strong evidence that the ultraviolet A (UVA) part of the solar spectrum contributes to the development of skin cancers. Its effect on the skin immune system, however, has not been fully investigated. Here, we analyzed the effects of UVA radiation on dermal dendritic cells (DDC), which, in addition, provided further characterization of these cells. Dermal sheets were obtained from normal human skin and irradiated, or not, with UVA at 2 or 12 J per cm(2). After a 2 d incubation, the phenotype of emigrant cells was analyzed by double immunostaining and flow cytometry. Results showed that migratory DDC were best characterized by CD1c expression and that only few cells co-expressed the Langerhans cell marker Langerin. Whereas the DC extracted from the dermis displayed an immature phenotype, emigrant DDC showed increased expression of HLA-DR and acquired co-stimulation and maturation markers. We showed here that UVA significantly decreased the number of viable emigrant DDC, a process related to increased apoptosis. Furthermore, UVA irradiation impaired the phenotypic and functional maturation of migrating DDC into potent antigen-presenting cells, in a concentration-dependent manner. The results provide further evidence that UVA are immunosuppressive and suggest an additional mechanism by which solar radiation impairs immune response.
引用
收藏
页码:1032 / 1038
页数:7
相关论文
共 39 条
[1]   High ultraviolet A protection affords greater immune protection confirming that ultraviolet A contributes to photoimmunosuppression in humans [J].
Baron, ED ;
Fourtanier, A ;
Compan, D ;
Medaisko, C ;
Cooper, KD ;
Stevens, SR .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2003, 121 (04) :869-875
[2]   UVA-induced immune suppression in human skin: Protective effect of vitamin E in human epidermal cells in vitro [J].
ClementLacroix, P ;
Michel, L ;
Moysan, A ;
Morliere, P ;
Dubertret, L .
BRITISH JOURNAL OF DERMATOLOGY, 1996, 134 (01) :77-84
[3]   ANTIGEN-PRESENTING OKM5+ MELANOPHAGES APPEAR IN HUMAN-EPIDERMIS AFTER ULTRAVIOLET-RADIATION [J].
COOPER, KD ;
NEISES, GR ;
KATZ, SI .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1986, 86 (04) :363-370
[4]   Broad-spectrum sunscreens provide greater protection against ultraviolet-radiation-induced suppression of contact hypersensitivity to a recall antigen in humans [J].
Damian, DL ;
Halliday, GM ;
Barnetson, RS .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1997, 109 (02) :146-151
[5]  
DEGRUIJL FR, 1993, CANCER RES, V53, P53
[6]   Ultraviolet AI exposure of human skin results in Langerhans cell depletion and reduction of epidermal antigen-presenting cell function: partial protection by a broad-spectrum sunscreen [J].
Dumay, O ;
Karam, A ;
Vian, L ;
Moyal, D ;
Hourseau, C ;
Stoebner, P ;
Peyron, JL ;
Meynadier, J ;
Cano, JP ;
Meunier, L .
BRITISH JOURNAL OF DERMATOLOGY, 2001, 144 (06) :1161-1168
[7]  
Duthie MS, 1999, BRIT J DERMATOL, V140, P995
[8]   Expression of C-type lectin receptors by subsets of dendritic cells in human skin [J].
Ebner, S ;
Ehammer, Z ;
Holzmann, S ;
Schwingshackl, P ;
Forstner, M ;
Stoitzner, P ;
Huemer, GM ;
Fritsch, P ;
Romani, N .
INTERNATIONAL IMMUNOLOGY, 2004, 16 (06) :877-887
[9]   CD1d is expressed on dermal dendritic cells and monocyte-derived dendritic cells [J].
Gerlini, G ;
Hefti, HP ;
Kleinhans, M ;
Nickoloff, BJ ;
Burg, G ;
Nestle, FO .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (03) :576-582
[10]   UVA-induced immunosuppression [J].
Halliday, GM ;
Bestak, R ;
Yuen, KS ;
Cavanagh, LL ;
Barnetson, RS .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1998, 422 (01) :139-145