Identification of sugarcane striate mosaic-associated virus by partial characterization of its double-stranded RNA

被引:8
作者
Choi, YG
Croft, BJ
Randles, JW
机构
[1] Univ Adelaide, Dept Appl & Mol Ecol, Glen Osmond, SA 5064, Australia
[2] Bur Sugar Expt Stn, Woodford, Qld 4514, Australia
[3] Univ Calif Riverside, Dept Plant Pathol, Riverside, CA 92521 USA
关键词
AMV; CF11; CMV; microgranular cellulose; phytoplasma; PSbMV; TMV;
D O I
10.1094/PHYTO.1999.89.10.877
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Sugarcane striate mosaic (ScSM)-affected sugarcane leaves contain a disease-associated 9-kilobase (kb) double-stranded RNA (dsRNA), usually together with 6- and 2.6-kb dsRNAs. The purified 9-kb dsRNA was amplified by the randomly primed polymerase chain reaction (PCR) and cloned. The nucleotide sequences of three separate regions, representing about 2.55 kb (28%) of the dsRNA sequence, were found to have significant similarities to viruses in the genera Capillo-, Carla-, Fovea-, Potex-, Poty-, Tricho-, and Tymovirus. Greatest overall similarity was found to apple stem pitting virus, with less similarity to blueberry scorch virus and potato virus M. A standard virus purification procedure was used to identify slightly flexuous filamentous particles that copurified with the disease-associated RNA. Particle modal lengths were approximately 950 and 1,900 nm with a diameter of 15 nm. Preparations contained a 51-kDa putative capsid protein and a 9-kb single-stranded RNA with a probable 3' polyadenylate tract. These ScSM-associated virus particles differ physically from viruses in existing genera because of their relative rigidity, length, and putative coat protein size. Reverse-transcription PCR with a primer pair designed from the sequenced segments amplified a 820-base pair fragment from ScSM-affected but not healthy sugarcane plants.
引用
收藏
页码:877 / 883
页数:7
相关论文
共 23 条
[1]  
BRUNT AA, 1995, VIRUS TAXONOMY, P475
[2]   Microgranular cellulose improves dsRNA recovery from plant nucleic acid extracts [J].
Choi, YG ;
Randles, JW .
BIOTECHNIQUES, 1997, 23 (04) :610-611
[3]  
CHOI YG, 1997, THESIS U ADELAIDE AD
[4]  
CHOI YG, 1995, P BIENN AUSTR PLANT, P84
[5]  
CROFT BJ, 1996, ACIAR P, V67, P55
[6]  
DODDS JA, 1993, DIAGNOSIS PLANT VIRU, P273
[7]  
Doyle J. J., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362
[8]  
DULIEU P, 1988, J VIROL METHODS, V24, P77
[9]   A RANDOM-PCR METHOD (RPCR) TO CONSTRUCT WHOLE CDNA LIBRARY FROM LOW AMOUNTS OF RNA [J].
FROUSSARD, P .
NUCLEIC ACIDS RESEARCH, 1992, 20 (11) :2900-2900
[10]   AN IMPROVED ALGORITHM FOR MATCHING BIOLOGICAL SEQUENCES [J].
GOTOH, O .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 162 (03) :705-708